Association of NQO1 C609T (Pro187Ser) with Risk of Oral Submucous Fibrosis in Eastern Indian Population

Authors

  • Sanjit Mukherjee CSIR-Indian Institute of Chemical Biology, Kolkata, India
  • Sweta Mohanty Department of Oral Pathology, Dr. R. Ahmed Dental College & Hospital, Kolkata, India
  • Atul Katarkar CSIR-Indian Institute of Chemical Biology, Kolkata, India. University of Lausanne, Epalinges-1066, Switzerland
  • Richa Dhariwal Department of Oral Pathology, Dr. R. Ahmed Dental College & Hospital, Kolkata, India
  • Basudev Mahato Department of Oral Pathology, Dr. R. Ahmed Dental College & Hospital, Kolkata, India
  • Jay Gopal Ray Department of Oral Pathology, Dr. R. Ahmed Dental College & Hospital, Kolkata, India
  • Keya Chaudhuri CSIR-Indian Institute of Chemical Biology, Kolkata, India.

Keywords:

Areca nut, Oral submucous fibrosis, NQO1, single nucleotide polymorphism, carcinogenesis.

Abstract

Objective: Oral submucous fibrosis (OSF) is a debilitating disease mainly attributed to chewing areca nut with a 7.4-13% malignant transformation rate. The present study explores the role of NADPH quinone oxidoreductase 1 (NQO1) C609T (Pro187Ser) polymorphism in susceptibility to OSF among habitual areca nut chewers in an eastern Indian population.
Material and Methods: In this hospital-based study, 152 controls and 179 OSF cases were genotyped at NQO1 C609T polymorphic site by PCR-RFLP and its effect on NQO1 expression in OSF tissue was studied to determine the risk of the disease.
Results: Overall, about 18% of the total OSF cases were detected carrying minor TT alleles (Ser/Ser) p=0.026. When categorized by age, both CT (Pro/Ser) and TT (Ser/Ser) alleles were significantly higher (p= 0.003 & 0.004 respectively) in cases above 40years of age. NQO1 protein was 42% reduced in buccal tissues of heterozygous (Pro/Ser) carriers, whereas a 70% reduction was observed in TT (Ser/Ser) OSF cases.
Conclusion: Our study suggests that the NQO1 C609T polymorphism confers increased risk for OSF in habitual chewers. 

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Published

2021-07-09

Issue

Vol. 6 No. 3 (2021)

Section

Research Articles/ Original Work

License

 West Asia Organization for Cabcer Prevention retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License 4 (This permits anyone to copy, distribute, transmit and adapt the published work, provided the original work and source are appropriately cited).