Synthesis, and Evaluation of a 4,4′-Methylenedianiline-Based Ligand and some Complexes as Against Colorectal Cancer

Abstract

Introduction: The researchers sought to create a new bis-azo ligand as a progression of their previous studies.

Materials and Methods: The ligand, designated as 6,6 (methylenebis (4,1-phenylene)) bis (diazene-2,1-diyl)) bis (2,4-dimethylphenol), was produced, followed by the preparation of a variety of chelate complexes with Pd+2, and Au+3 ions. The characterization of these compounds was performed using advanced techniques such as elemental analysis, FT-IR spectroscopy, H, NMR spectroscopy, thermal analysis, through DSC to elucidate the synthesized complexes.

Results: Analysis indicated that the complexes produced with Pd+2, and Au+3 ions demonstrated a 2:1 metal-ligand ratio. The ligand was identified as bidentate (N-O) in nature. Molecular docking methods were employed to assess the effectiveness of these compounds in cancer treatment. The cytotoxicity of the 6-MPDDB dye ligand and its palladium (II) complex in colorectal carcinoma was assessed Using the MTT method. The dye and its metal complexes were evaluated for their antioxidant activity by assessing their ability to scavenge free radicals using the DPPH assay, with ascorbic acid serving as the standard. The IC₅₀ values were determined, revealing that the ligand demonstrated notable free radical inhibition, whereas the antioxidant potential of the complexes differed according to their respective IC₅₀ values.

Conclusion: The synthesized ligand and its Pd(II) and Au(III) complexes demonstrated well-defined structural characteristics and promising biological activities. Overall, the results suggest their potential as effective antioxidant and anticancer agents, warranting further investigation.