Low PD-1 mRNA Expression in Peripheral Blood of Childhood Leukemia

Authors

  • Mururul Aisyi Department of Paediatric Oncology, National Cancer Center-Dharmais Cancer Hospital, Jakarta, Indonesia.
  • Muhammad Al Azhar Functional Medical Staff of Surgical Oncology Department, Dharmais Hospital-National Cancer Center, Jakarta, Indonesia.
  • Fifi Dwijayanti Clinical Research Unit, Dharmais Hospital-National Cancer Center, Jakarta, Indonesia.
  • Ulfa Faujiah Department of Paediatric Oncology, National Cancer Center-Dharmais Cancer Hospital, Jakarta, Indonesia.

Keywords:

PD-1, pediatric leukemia, immunotherapy, immune checkpoint inhibitor

Abstract

Background:  The expression of PD-1 has been linked to prognosis and response to immune checkpoint inhibitors in solid tumors, but less is known about their role in pediatric blood cancers. Therefore, we aimed to study the levels of PD-1 mRNA in children with leukemia and explore how they relate to clinical factors.

Methods: Blood samples were collected from 15 children with leukemia and 11 healthy individuals. PD-1 mRNA expression was analyzed using real time PCR.

Results: PD-1 mRNA expression was significantly lower in childhood leukemia patients (average DCT: 12.3±2.5) compared to healthy individuals (average DCT: 10.3±0.5) (p=0.011). PD-1 mRNA expression did not correlate with gender, age, diagnosis, remission status, down syndrome, hyperleukocytosis, infection, and outcome.

Conclusion: Our findings indicated that mRNA expression of PD-1 was reduced in children with leukemia compared to healthy individuals and was not associated with all analyzed clinical factors. These results provide early insights into PD-1 expression in Indonesian pediatric leukemia patients.

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Published

2025-01-12

Issue

Vol. 10 No. 1 (2025)

Section

Research Articles/ Original Work

License

 West Asia Organization for Cabcer Prevention retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License 4 (This permits anyone to copy, distribute, transmit and adapt the published work, provided the original work and source are appropriately cited).