Capacity of Thymidylate Synthase and Dihydropyrimidine Dehydrogenase mRNA Expression to Predict Neoadjuvant Chemotherapy Response of 5fu Regimen in Advanced Colorectal Cancer: A Cross-Sectional Study

Authors

  • Elvida Christi Imelda Tahiya Division of Digestive Surgery, Department of Surgery, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia. https://orcid.org/0009-0009-3777-9947
  • Ronald Erasio Lusikooy Division of Digestive Surgery, Department of Surgery, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia. Dr. Wahidin Sudirohusodo Hospital, Makassar, Indonesia. Hasanuddin University Hospital, Makassar, Indonesia. https://orcid.org/0000-0001-5015-4583
  • Ilhamjaya Patelongi Department of Physiology, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia. https://orcid.org/0000-0002-1100-4360
  • Warsinggih Warsinggih Division of Digestive Surgery, Department of Surgery, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia. Dr. Wahidin Sudirohusodo Hospital, Makassar, Indonesia. Hasanuddin University Hospital, Makassar, Indonesia. https://orcid.org/0000-0002-1756-9039
  • Julianus Aboyaman Uwuratuw Division of Digestive Surgery, Department of Surgery, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia. https://orcid.org/0000-0002-7116-6628
  • Andi Asadul Islam Department of Neurosurgery, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia.
  • Muhammad Faruk Hasanuddin University Hospital, Makassar, Indonesia. Department of Surgery, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia. https://orcid.org/0000-0002-7079-4585

Keywords:

Colorectal cancer, chemotherapy, predictor, thymidylate synthase, dihydropyrimidine dehydrogenase

Abstract

Background: Chemotherapy is an important component of colorectal cancer treatment. Despite its success in inducing tumor cell death, chemotherapy has been constrained by resistance and adverse side effects. Therefore, predictive markers are required to ensure that the appropriate chemotherapy is administered based on the individual patient. This study aimed to correlate the mRNA expression of the TS and DPD genes with the tumor and CEA responses to the 5FU chemotherapy regimen.

Methods: This was a cross-sectional study with a prognostic test design. Measurements of tumor mass and tissue sampling were done prior to chemotherapy. CT scans were performed to measure tumor size pre-chemotherapy and after two cycles of treatment with a lag time of three weeks. The mRNA expression of the TS and DPD genes was then evaluated in each group.

Results: The mean value of the mRNA expression of the TS gene (9.555±1.693) showed an insignificant correlation with clinical response p=0.195 (p<0.05). However, the mean value of the mRNA expression of the DPD gene (7.461±1.088) was significantly correlated with clinical response p=0.003 (p<0.05).

Conclusion: DPD mRNA can be considered a marker of chemotherapy responsiveness when choosing a chemotherapy regimen. 

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Published

2025-08-23

Issue

Vol. 10 No. 3 (2025)

Section

Research Articles/ Original Work

License

 West Asia Organization for Cabcer Prevention retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License 4 (This permits anyone to copy, distribute, transmit and adapt the published work, provided the original work and source are appropriately cited).